KC-5732

MC38-CDH17-GFP-Luc2-Low Cell Line

59560

Home » MC38-CDH17-GFP-Luc2-Low Cell Line

Background of MC38-CDH17-GFP-Luc2-Low Cell Line

CDH17, also known as HPT1, is a protein that belongs to the cadherin family of calcium-dependent cell adhesion molecules. CDH17 is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. CDH17 may also play a role in the morphological organization of liver and intestine.

Specifications

Catalog Number	KC-5732
Cell Line Name	MC38-CDH17-GFP-Luc2-Low Cell Line
Clone Number	7#
Host Cell Line	MC38
Description	Stable MC38 cell line expressing exogenous CDH17 GFP Luc2 gene in low level.
Quantity	Two vials of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% DMEM + 20% FBS + 10% DMSO
Propagation Medium	DMEM + 10% FBS
Selection Marker	NA
Morphology	Epithelial
Subculture	Split saturated culture 1:4~1:8 every 2~3 days; seed out at about 1-2 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative

Cell Line Generation

MC38 CDH17 GFP Luc2 Cell Line was generated using a lentiviral vector expressing the CDH17 GFP Luc2 sequence.

Characterization

Figure 1: Characterization of CDH17 GFP Luc2 overexpression in the MC38 CDH17 GFP Luc2 stable clone using FACS.

Figure 2: Characterization of luciferase expression in MC38 CDH17 GFP Luc2 stable clone using the Bright-Lite Luciferase Detection System.

Figure 3: Characterization of CDH17 GFP Luc2 overexpression in the MC38 CDH17 GFP Luc2 stable clone using PCR sequence.

Cell Resuscitation

1. Prewarm culture medium (DMEM supplemented with 10% FBS)in a 37°C water bath.
2. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
3. Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
4. Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
5. Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
6. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
7. Incubate the flask at 37°C, 5% CO₂ incubator.
8. Split saturated culture 1:4~1:8 every 2~3 days; seed out at about 1-2 × 10⁵ cells/mL.

Cell Freezing

1. Prepare the freezing medium (70% DMEM + 20% FBS + 10% DMSO) fresh immediately before use.
2. Keep the freezing medium on ice and label cryovials.
3. Transfer cells to a sterile, conical centrifuge tube, and count the cells.
4. Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
5. Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
6. Aliquot 1 mL of the cell suspension into each cryovial.
7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
8. Transfer vials to liquid nitrogen for long-term storage.

References

1. Kremmidiotis G, Baker E, Crawford J, Eyre HJ, Nahmias J, Callen DF (Aug 1998). "Localization of human cadherin genes to chromosome regions exhibiting cancer-related loss of heterozygosity". Genomics. 49 (3): 467–71.
2.Chalmers IJ, Hofler H, Atkinson MJ (Jun 1999). "Mapping of a cadherin gene cluster to a region of chromosome 5 subject to frequent allelic loss in carcinoma". Genomics. 57 (1): 160–3.