KC-2182-DW

MC38-FGFR2B-High Cell Line

57636

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Background of MC38-FGFR2B-High Cell Line

FGFR2B is an epithelial-specific isoform of fibroblast growth factor receptor 2, generated by alternative splicing of the FGFR2 gene. It primarily binds to mesenchymal-derived FGF ligands (e.g., FGF7, FGF10) and plays critical roles in embryonic development, tissue repair, and epithelial homeostasis. In oncology, aberrant activation of FGFR2B—through overexpression, amplification, or ligand-dependent signaling—drives tumor progression in multiple cancers, particularly gastric, breast, and endometrial carcinomas. Its restricted expression pattern and ligand specificity make it a compelling therapeutic target, with inhibitors currently developed to block hyperactivated FGFR2B pathways in precision medicine.

Specifications

Catalog Number	KC-2182-DW
Cell Line Name	MC38-FGFR2B-High Cell Line
Clone Number	2#
Host Cell Line	MC38
Description	Stable MC38 cell line expressing exogenous FGFR2B gene
Quantity	Two vials of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% DMEM+20% FBS+10% DMSO
Propagation Medium	DMEM+10% FBS +5μg/mL Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Subculture	Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative

Cell Line Generation

MC38-FGFR2B-High cell line was generated using lentivirus expressing FGFR2B sequence.

Characterization

Figure 1. Characterization of FGFR2B over-expression in the MC38-FGFR2B-High stable clone using FACS.[Primary antibody: Bemarituzumab(FGFR2b), Cat#KA-1202, Kyinno]

Figure 2:Validation of in vivo tumorigenicity of MC38 cells stably expressing FGFR2B via subcutaneous implantation in C57BL/6J mice, with tumor growth monitored by measuring volume (V=0.5×length×width²) and body weight.

Cell Resuscitation

Prewarm culture medium (DMEM supplemented with 10% FBS, 5μg/mL Puromycin)in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (70% DMEM + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage.

References

Tartaglia M, Valeri S, Velardi F, Di Rocco C, Battaglia PA. Trp290Cys mutation in exon IIIa of the fibroblast growth factor receptor 2 (FGFR2) gene is associated with Pfeiffer syndrome. Hum Genet. 1997 May;99(5):602-6. doi: 10.1007/s004390050413. PMID: 9150725.