KC-5508

MC38-CD13 Cell Line

56972

Home » MC38-CD13 Cell Line

Background of MC38-CD13 Cell Line

a receptor for coronaviruses. CD13 functions in cell surface antigen expression by trimming the N-terminal amino acids of MHC class II-bound peptides. CD13 is also a myeloid antigen that can be used for diagnosing acute myeloid leukemia and granulocytic sarcoma. CD13 can also be used to distinguish Down syndrome-related acute myeloid leukemia from transient myeloproliferative disorders.

Specifications

Catalog Number	KC-5508
Cell Line Name	MC38-CD13 Cell Line
Host Cell Line	MC38
Description	Stable MC38 cell line expressing exogenous CD13 gene
Quantity	One vial of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% RPMI1640 + 20% FBS + 10% DMSO
Propagation Medium	RPMI1640 + 10% FBS + 5μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Subculture	Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/ml
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative

Cell Line Generation

MC38-CD13 Cell Line was generated using a lentiviral vector expressing the CD13 sequence.

Characterization

Figure 1: Characterization of CD13 overexpression in the MC38-CD13 stable clone using FACS.

Figure 2: Characterization of human CD13 overexpression in the MC38-CD13 stable clone using PCR sequence.

Cell Resuscitation

1. Prewarm culture medium (RPMI1640 supplemented with 10% FBS and 5μg/mL Puromycin)in a 37°C water bath.
2. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
3. Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
4. Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
5. Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
6. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
7. Incubate the flask at 37°C, 5% CO₂ incubator.
8. Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

1. Prepare the freezing medium (RPMI1640 + 20% FBS + 10% DMSO) fresh immediately before use.
2. Keep the freezing medium on ice and label cryovials.
3. Transfer cells to a sterile, conical centrifuge tube, and count the cells.
4. Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
5. Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
6. Aliquot 1 mL of the cell suspension into each cryovial.
7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
8. Transfer vials to liquid nitrogen for long-term storage

References

1. Lu C, Amin MA, Fox DA. CD13/Aminopeptidase N Is a Potential Therapeutic Target for Inflammatory Disorders. J Immunol. 2020 Jan 1;204(1):3-11. doi:10.4049/jimmunol.1900868. PMID: 31848300; PMCID: PMC6997018.
2. Guo Q, Li X, Cui MN, Sun JL, Ji HY, Ni BB, Yan MX. CD13: A Key Player in Multidrug Resistance in Cancer Chemotherapy. Oncol Res. 2020 Dec10;28(5):533-540. doi: 10.3727/096504020X15919605976853. Epub 2020 Jun 12. PMID:32532363; PMCID: PMC7751223.
3. Ghosh M, Kelava T, Madunic IV, Kalajzic I, Shapiro LH. CD13 is a criticalregulator of cell-cell fusion in osteoclastogenesis. Sci Rep. 2021 May24;11(1):10736. doi: 10.1038/s41598-021-90271-x. PMID: 34031489; PMCID:PMC8144195.

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.