KC-2175

MC38-mouse-MMP7-Cell-Line

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Background of MC38-mouse-MMP7-Cell-Line

MMP-7, also named MPSL1, is a small proteolytic enzyme that secretes zinc and calcium endopeptidases. It can degrade a variety of extracellular matrix substrates and other substrates and plays important regulatory roles in many human pathophysiological processes. This gene exhibits elevated expression levels in multiple human cancers, especially the digestive cancers.

Specifications

Catalog Number	KC-2175
Cell Line Name	MC38-mouse-MMP7-Cell-Line
Host Cell Line	Mouse MC38 cell line
Description	Stable MC38 clone expressing exogenous mouse MMP7 gene
Quantity	Two vials of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% RPMI1640+20% FBS+10% DMSO
Propagation Medium	RPMI1640+10%FBS
Morphology	Epithelial
Subculture	Split saturated culture 1:2-1:5 every 3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 48 hours
Mycoplasma Status	Negative
In Vivo Validation	NA

Cell Line Generation

MC38 mouse MMP7 cell Line was generated using a retroviral vector expressing mouse MMP7 sequence.

Characterization

Cell Resuscitation

Prewarm culture medium (RPMI1640 + 10%FBS) in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:2-1:5 every 3 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (70% RPMI-1640 + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage

References

Scheau C, Badarau IA, Costache R, et al. The Role of Matrix Metalloproteinases in the Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma. Anal Cell Pathol (Amst). 2019;2019:9423907. Published 2019 Nov 26.
Roles of matrix metalloproteinase-7 (MMP-7) in cancer.Liao HY, Da CM, Liao B, Zhang HH.Clin Biochem. 2021 Jun;92:9-18. Epub 2021 Mar 10.
Association between promoters polymorphisms of matrix metalloproteinases and risk of digestive cancers: a meta-analysis.Li X, Qu L, Zhong Y, Zhao Y, Chen H, Daru L.J Cancer Res Clin Oncol. 2013 Sep;139(9):1433-47. Epub 2013 May 5.