KC-1528

MCF7-Tet-off-GFP-Cell-Line

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Home » 细胞系 » MCF7-Tet-off-GFP-Cell-Line

Background of MCF7-Tet-off-GFP-Cell-Line

Specifications

Catalog NumberKC-1528
Cell Line NameMCF7-Tet-off-GFP-Cell-Line
Host Cell LineHuman MCF7 cell line
DescriptionStable MCF7 clone expressing exgenous GFP under the control of tet-off system
QuantityTwo vials of frozen cells (≥2-106/vial)
StabilityStable in culture over a minimum of 10 passages
ApplicationDrug screening and biological assays
Freezing Medium70% RPMI1640+20% FBS+10% DMSO
Propagation MediumRPMI1640+10%FBS+1mg/mL G418+200µg/mL Hygromycin B
Selection MarkerHygromycin B
Incubation37 °C with 5% CO2
StorageLiquid nitrogen immediately upon receiving
Mycoplasma StatusNegative
In Vivo ValidationNA

Cell Line Generation

Characterization

Cell Resuscitation

Cell Freezing

References

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