KC-1957

MDA-MB-231-PDL1-KO-PD1v-CD81-GFP-Cell-Line

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Home » 细胞系 » MDA-MB-231-PDL1-KO-PD1v-CD81-GFP-Cell-Line

Background of MDA-MB-231-PDL1-KO-PD1v-CD81-GFP-Cell-Line

Specifications

Catalog NumberKC-1957
Cell Line NameMDA-MB-231-PDL1-KO-PD1v-CD81-GFP-Cell-Line
Host Cell LineHuman MDA-MB-231 cell line
DescriptionStable MDA-MB-231 clone with PDL1 gene knockout, and expressing exogenous human PD1v mutant, CD81 and GFP
QuantityTwo vials of frozen cells (≥2-106/vial)
StabilityStable in culture over a minimum of 10 passages
ApplicationDrug screening and biological assays
Freezing Medium70% RPMI1640+20% FBS+10% DMSO
Propagation MediumRPMI1640+10%FBS+1µg/mL Puromycin+100µg/mL Hygromycin B
Selection MarkerPuromycin
Incubation37 °C with 5% CO2
StorageLiquid nitrogen immediately upon receiving
Mycoplasma StatusNegative
In Vivo ValidationNA

Cell Line Generation

Characterization

Cell Resuscitation

Cell Freezing

References

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