KB-1606

MK3475-hIgG1

45988

Background of MK3475-hIgG1

Pembrolizumab is a humanized monoclonal antibody therapy that targets the cell surface receptor PD-1. It is an immune checkpoint inhibitor initially approved in 2015 for treating metastatic non-small cell lung cancer (NSCLC). Pembrolizumab is an approved first-line therapy for unresectable metastatic melanoma that is often continued beyond complete response despite the fact that immune-related toxicities gradually increase with continuation of therapy.

Specifications

Catalog Number	KB-1606
Antibody Name	MK3475-hIgG1
Isotype	Human IgG1,kappa
Target	PD1
Species Reactivity	Human
Host Cell Line	EXPI CHO
Purification Method	Affinity purified
Concentration	>2 mg/mL
Formulation	50 mM sodium citrate,150mM NaCl,pH5.5
Purity	>95% by SDS-PAGE and SEC-HPLC
Validation	ELISA
Endotoxin Level	<0.2 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	It is recommended that the protein should be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

Garon EB, Rizvi NA, Hui R, et al. Pembrolizumab for the treatment of non–small-cell lung cancer. N Engl J Med 2015;372:2018-2028.
Chatterjee M, Turner DC, Felip E, et al. Systematic evaluation of pembrolizumab dosing in patients with advanced non-small-cell lung cancer. Ann Oncol 2016;27:1291-1298.