KB-1278

PY314-hIgG1

45419

Background of PY314-hIgG1

PY314 is an anti-TREM2 monoclonal antibody for the treatment of advanced solid tumors. Binding leads to targeted depletion of TREM2-positive cells through processes called antibody-dependent cell mediated cytotoxicity (ADCC) or antibody-dependent cell-mediated phagocytosis (ADCP). When TAMs are depleted from tumors, pro-inflammatory, anti-tumor immune cells, such as CD8 T-cells, natural killer (NK) and M1-like macrophages, become activated and penetrate tumors leading to tumor destruction.

Specifications

Catalog Number	KB-1278
Antibody Name	PY314-hIgG1
Isotype	Human IgG1,kappa
Target	TREM2
Species Reactivity	Human
Host Cell Line	EXPI CHO
Purification Method	Affinity purified
Concentration	>2 mg/mL
Formulation	50 mM sodium citrate,150mM NaCl,pH5.5
Purity	>95% by SDS-PAGE and SEC-HPLC
Validation	ELISA \| FACS
Endotoxin Level	<0.2 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	It is recommended that the protein should be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

Guerreiro RJ, Lohmann E, Bras JM, Gibbs JR, Rohrer JD, Gurunlian N, et al. Using exome sequencing to reveal mutations in TREM2 presenting as a frontotemporal dementia-like syndrome without bone involvement. JAMA Neurol. 2013a;70:78–84.
Guerreiro R, Wojtas A, Bras J, Carrasquillo M, Rogaeva E, Majounie E, et al. TREM2 variants in Alzheimer's disease. N Engl J Med. 2013b;368:117–27.
Hsieh CL, Koike M, Spusta SC, Niemi EC, Yenari M, Nakamura MC, et al. A role for TREM2 ligands in the phagocytosis of apoptotic neuronal cells by microglia. J Neurochem. 2009;109:1144–56.