KB-1144

REGN7075-CD28-hIgG4-S228P

12149

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Background of REGN7075-CD28-hIgG4-S228P

CD28 is a direct receptor of superantigen toxins broadens the scope of microbial pathogen recognition mechanism. CD28 plays a critical role in regulating immune responses both by enhancing effector T cell activation and differentiation and controlling the development and function of regulatory T cells. CD28 stimulation leads to recruitment of a beta-arrestin/phosphodiesterase-4 (PDE4) complex to rafts, resulting in down-regulation of cAMP levels.

Specifications

Catalog Number	KB-1144
Antibody Name	REGN7075-CD28-hIgG4-S228P
Isotype	Human IgG4-S228P, kappa
FC Muations	S228P
Target	CD28
Species Reactivity	Human
Host Cell Line	EXPI CHO
Purification Method	Affinity purified
Concentration	>2 mg/mL
Formulation	50 mM sodium citrate,150mM NaCl,pH5.5
Purity	>95% by SDS-PAGE and SEC-HPLC
Validation	FACS
Endotoxin Level	<0.2 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	It is recommended that the protein should be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

Kaempfer R, Arad G, Levy R, Hillman D, Nasie I, Rotfogel Z. CD28: direct and critical receptor for superantigen toxins. Toxins (Basel). 2013 Sep 9;5(9):1531-42.
Leddon SA, Fettis MM, Abramo K, Kelly R, Oleksyn D, Miller J. The CD28 Transmembrane Domain Contains an Essential Dimerization Motif. Front Immunol. 2020 Jul 16;11:1519.
Bjørgo E, Taskén K. Novel mechanism of signaling by CD28. Immunol Lett. 2010 Mar 10;129(1):1-6.