KB-1097

RG6333-CD28-hIgG4-S228P

12111

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Background of RG6333-CD28-hIgG4-S228P

CD28 signals play a key role in many T cell processes, including cytoskeletal remodeling, production of cytokines, survival, and differentiation. A strong CD28 signal, thus, indicates to the T cell that there is indeed a threat and that an immune response is urgently required. CD28 provides signals during T cell activation which are required for production of interleukin 2 and other cytokines and chemokines, and it has also been implicated in the regulation of T cell anergy and programmed T cell death.

Specifications

Catalog Number	KB-1097
Antibody Name	RG6333-CD28-hIgG4-S228P
Isotype	Human IgG4-S228P, kappa
FC Muations	Wild Type
Target	CD28
Species Reactivity	Human
Host Cell Line	EXPI CHO
Purification Method	Affinity purified
Concentration	>2 mg/mL
Formulation	50 mM sodium citrate,150mM NaCl,pH5.5
Purity	>95% by SDS-PAGE and SEC-HPLC
Validation	ELISA \| FACS
Endotoxin Level	<0.2 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	It is recommended that the protein should be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

Esensten JH, Helou YA, Chopra G, Weiss A, Bluestone JA. CD28 Costimulation: From Mechanism to Therapy. Immunity. 2016 May 17;44(5):973-88.
Beyersdorf N, Kerkau T. CD28-Kostimulation und Checkpointblockade in T-Zellen [CD28 costimulation and checkpoint inhibition in T cells]. Internist (Berl). 2020 Jul;61(7):652-659. German.
Ward SG. CD28: a signalling perspective. Biochem J. 1996 Sep 1;318 ( Pt 2)(Pt 2):361-77.