KC-2397

RM1-FOLH-Luc2-Cell-Line

23976

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Background of RM1-FOLH-Luc2-Cell-Line

FOLH, also known as PSMA, is a type II transmembrane glycoprotein belonging to the M28 peptidase family, which is a glutamate-preferrng carboxy-peptidase. PSMA is highly expressed in prostate secretory-acinar epithelium, in some benign extraprostatic epithelia cellsfrom breast, duodenum, and kidney tissues, and in prostate cancer. In the prostate the protein is up-requlated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer.

Specifications

Catalog Number	KC-2397
Cell Line Name	RM1-FOLH-Luc2-Cell-Line
Host Cell Line	Mouse RM1 cell line
Description	Stable RM1 cell line expressing exogenous FOLH gene
Quantity	One vial of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% RPMI1640+20% FBS+10% DMSO
Propagation Medium	RPMI1640+10%FBS+2μg/mL Puromycin
Selection Marker	Puromycin
Morphology	Fibroblast-like
Subculture	Split saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative
In Vivo Validation	Yes

Cell Line Generation

RM1 Human FOLH Luc2 cell line was generated using a lentiviral vector expressing the human FOLH sequence.

Characterization

Cell Resuscitation

Prewarm culture medium (RPMI1640 + 10% FBS + 2μg/mL Puromycin) in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (70% RPMI-1640 + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage

References

Prostate-Specific Membrane Antigen (PSMA) Theranostics for Treatment of Oligometastatic Prostate Cancer.PMID: 34829977 PMCID: PMC8621856 DOI: 10.3390/ijms222212095
GCPII imaging and cancer. C A Foss 1, R C Mease, S Y Cho, H J Kim, M G Pomper.PMID: 22304713 PMCID: PMC4076792 DOI: 10.2174/092986712799462612
Is prostate-specific membrane antigen a multifunctional protein Ayyappan? K Rajasekaran 1, Gopalakrishnapillai Anilkumar, Jason J Christiansen.PMID: 15840561 DOI: 10.1152/ajpcell.00506.2004

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.