| Catalog Number | KC-0152 |
|---|
| Cell Line Name | U2OS-REV-GFP-Cell-Line |
|---|
| Host Cell Line | Human U2OS cell line |
|---|
| Description | Stable U2OS clone expressing exogenous human GFP tagged REV |
|---|
| Quantity | Two vials of frozen cells (≥2-106/vial) |
|---|
| Stability | Stable in culture over a minimum of 10 passages |
|---|
| Application | Drug screening and biological assays |
|---|
| Freezing Medium | 70% McCoy's 5a+20%FBS+10%DMSO |
|---|
| Propagation Medium | McCoy's 5a+10%FBS+2µg/mL Puromycin |
|---|
| Selection Marker | Puromycin |
|---|
| Subculture | Split saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 105 cells/mL |
|---|
| Incubation | 37 °C with 5% CO2 |
|---|
| Storage | Liquid nitrogen immediately upon receiving |
|---|
| Mycoplasma Status | Negative |
|---|
| In Vivo Validation | NA |
|---|
